"Rational Design" can sound like a throw away buzzword, but

The MODERN VECTOR represents a new vector synthesis.

The basic premise: every feature, every choice, was made with a plan for the future. 

As a molecular biologist, you know bacteria have an, (unofficial), maximum sequence size for vectors they will consistently amplify.  While it heavily depends on the sequence involved, the rough limit is 10-12Kb.  While that may sound large, with modern desires to put in more than one open reading frame (ORF) using 2a peptides or IRES sequences and other expression modification components, it can be quite easy to reach that limit.

Many commercially available legacy vectors contain sequence that was useful at one point in time, but due to technological improvements, (such as PCR), this legacy sequence is now obsolete.

It is only 30% larger than pUC19, but there is considerable functionality packed into that 30%.

The MODERN VECTOR has the capacity to handle more of what you want.

Like many molecular biologists, you may want to make changes to the vector you are using. Perhaps you have some proprietary sequence that you want to add, or maybe you want to change the main promoter, poly A signal, whatever. Legacy vectors can make that difficult, either by having no restriction sites where you need them, or multiple sites in inappropriate locations. The MODERN VECTOR was designed to make it easier than ever to build the vector YOU want.  Deliberate placement of unique restriction sites allow major features to be modified.  But more than that, the small size gives you the flexibility to add customizations that make your work easier.

Unleash your creativity with the MODERN VECTOR. 

When pBR322 was first realized in the 1970's, our understanding of vectors and bacterial hosts was quite limited.  There were few antibiotics available and even fewer resistance enzymes.

Since vectors were designed to be amplified in bacteria, it has become commonplace to have a bacterial selection marker run off a separate expression cassette.  However, as we push our plasmids to contain more sequence, more ORFs, more, more, more, that bacteria only resistance cassette has remained untouched, carried along with so many other legacy vector sequences.

It doesn't have to be this way.

We were able to create a novel promoter that has activity in both bacterial and eukaryotic hosts.  Through careful testing of a variety of antibiotics, we identified several that allowed selection in both.  Thus, each MODERN VECTOR has only a single, universal selection marker.

With genomic editing knock in experiments, unnecessary sequence needs to be minimized to reduce mistargeting and potential deleterious effects.  By eliminating an entire expression cassette, the MODERN VECTOR is sleek and efficient, earning its name "steamline".  Removing the bacteria only resistance cassette also freed up considerable space in the vector.

Two benefits, with one, long overdue, modification.  That's a MODERN VECTOR. 

 

Protein expression is the main reason for DNA vectors.  The ability to create recombinant protein is powerful and creates many opportunities.  While there are benefits for expressing protein in different hosts, legacy vectors forced you to use different constructs for each host making it time consuming just to get started.

The MODERN VECTOR has made that much easier.  For the main expression cassette, there are several strong, eukaryotic promoters and an optimally placed T7 bacteriophage promoter driving expression.  In this way you have the freedom to express your protein in either prokaryotes, mammalian cells/animals, or even in vitro with a variety of transcription/translation kits.  As in vitro expression becomes more advanced be sure that your vector is ready to harness those advances.

One vector, so many ways to use it.  That's a MODERN VECTOR.

The legacy vectors never anticipated genomic editing, so easy ways to add homology arm sequence were not part of the design.  But with genomic editing so commonplace, this limitation presents unexpected challenges.

Homology arms can add 1-3Kb (or more) of sequence.  On top of that they need to be optimally positioned so that only the desired sequence is added during "knock in".

However, it isn't just homology arms that might be added.  It could be viral LTRs, or chromosome opening sequences, or a whole host of other expression modification sequences.  The list is endless.  Good thing we added convenient restriction sites to add just about anything you want on either side of the expression cassettes.

Anticipating your needs, making your work easier.  That's a MODERN VECTOR.