pDKStreamline6-Puro (Metallothionein promoter)

pDKStreamline6-Puro is a compact vector with the Puromycin resistance marker.  While optimized for protein expression, this vector can be used as a shuttle vector.

Features Include:

• Small size – backbone around 3kb
• Inducible expression – The Metallothionein promoter is a native promoter that is inducible through the addition of heavy metals, such as Zinc, to the culture medium.  No other DNA binding proteins (such as artificial steroid receptors) are required for expression and induction.
• Easy insertion of homology arms – rationally designed restriction sites allow for the easy insertion of homology arms for genomic targeting and/or viral LTRs for easy virus assembly
• Minimized vector backbone – reduces or eliminates unneeded sequence to be included in your expression system.
• Resistance to Puromycin is expressed in both prokaryotes and eukaryotes.
• Patent Pending

NOTE:  The XbaI site is NOT blocked by methylation.